雌激素受體ERβ配體結合區的異源表達與純化

Heterologous expression and purification of the ligand binding domain of estrogen receptor β

摘要: 為研究環境化合物與雌激素受體的相互作用,在大腸桿菌中異源表達了人雌激素受體ERβ的配體結合區(hERβ-LBD)蛋白。在誘導劑(IPTG)濃度為0.2mmol·L^-1,培養溫度25℃,誘導時間為8h的優化表達條件下,經親和色譜法純化后,hERβ(LBD蛋白的產量可達236mg·L^-1.

Abstract: In order to investigate the interaction between environmental chemicals and estrogen receptors,the ligand binding domain of human estrogen receptor β(hERβ-LBD) was expressed in Escherichia coli.Based on the optimized expression conditions(IPTG,0.2 mmol·L^-1;culture temperature,25℃;time of induction,8 h),after purified by affinity chromatography,the production of hERβ-LBD can be reached to 236 mg·L^-1.